Pharmacological Assessment of Morinda CitrifoliaFruit Extract’s in Anti-Arthritic Properties in Wistar Rats
DOI:
https://doi.org/10.52783/jns.v14.1768Keywords:
Morinda citrifolia, Anti-arthritic activity, Freund’s adjuvant induced, Phytochemical analysis, In vitro antioxidant activityAbstract
Arthritis and related disorders, including rheumatoid arthritis (RA), are common diseases affecting millions of people. RA is characterized by articular injuries having an inflammatory propagation of synovial cells, attaining a nearly complete functional defect. It affects about 1% of the general population.Morinda citrifolia L(Rubiaceae), commonly called Noni or Indian mulberry, is a small evergreen tree or shrub of Polynesian origin. M. citrifolia bears a lumpy, green to yellowish-white fruit, normally 5 to 10 cm in length, with a surface covered in polygonal-shaped sections,The leaves, roots, bark and fruits have all been used medicinally to treat a wide range of ailments. These include, but are not limited to, diabetes, diarrhoea, hypertension, malaria, pain, and topical infections. The fruits are also eaten as a food, but primarily only in times of famine. The present study was designed to investigate anti-arthritic activity of methanolic fruits extract of M. citrifolia in Freund’s complete adjuvant (FCA)-induced arthritis in rats. Qualitative analysis of various phytochemical constituents was determined by the well-known test protocol available in the literature. The In vitro antioxidant activity of methanolic extract of the fruits was assessed against DPPH free radical scavenging assay method using standard protocols. M. citrifolia was evaluated for anti-arthritic action by Freund’s adjuvant induced arthritis test in adult Albino rats (200±20gm). Rats were injected 0.1 ml of complete Freund’s adjuvant into the planter region of the left hind paw. M. citrifolia extracts (200 and 400 mg/kg. b.wt.) was given orally to arthritic rats induced with Complete Freund’s Adjuvant and changes in rat paw volume and body weight was determined. Phytochemical analysis of methanolic extract of M. citrifolia showed the presence of carbohydrate, alkaloids, phenolics, tannin, triterpenoids, steroids, flavonoids and glycoside. In the oral acute toxicity studies, M. citrifolia was found to be safe as it did not cause any mortality up to 2000 mg/kg. Hence, 200 and 400 mg/kg doses were selected for the present study.DPPH radical scavenging activity of M. citrifolia extract exhibited percent inhibition 60.15% and its IC 50value was found to be 57.10μg/ml. Ascorbic was used as a reference compound which exhibited percent inhibition 86.36% and showed IC 50 value of 19.36μg/ml. M. citrifolia administered groups showed marked reduction in paw volume when compared with the negative control group (Group II). It was also found that there was significant weight loss when compared to standard. The results reveal promising anti-arthritic potential of the M. citrifolia. However further pharmacological investigation using isolated active ingredients can be carried out to confirm its efficacy and mechanism of action.
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Liu M, Dong J, Yang Y, Yang X, Xu H. Anti-inflammatory effects of triptolide loaded poly (D, L-lactic acid) nanoparticles on adjuvant induced arthritis in rats. J Ethnopharmacol 2005; 97:219-25.
Ward JR, Cloud RS. Comparative eff ect of antirheumatic drugs on adjuvant induced polyarthritis in rats. J Pharmacol Exp Ther 1966; 152:116-21.
Balasubramanian A, Manivannan R, Paramaguru R, Mazudmer PM, Vijayakumar M. Evaluation of anti-inflammatory and antioxidant activities of stem bark of Toddalia asiatica (L.) Lam using different experimental models. Pharmacologia 2012; 3:144-9.
Kirtikar K, Basu B (1933): Indian Medicinal Plants, Vol. II. Delhi, Periodical Expert Book Agency, pp. 1295.
Wang M, West B, Jensen C, Nowicki D, Su C, Palu A, Anderson G (2002): Morinda citrifolia (noni): A literature review and recent advances in noni research. Acta Pharmacol Sin 23: 1127–1141.
Nayak S, Mengi S. Immunostimulant activity of noni (Morinda citrifolia) on T and B lymphocytes. Pharmaceutical biology. 2010; 48(7):724-31.
Palu A, Hirazumi A, West B, Deng S, Jensen J, White L (2008): The effects of Morinda citrifolia L. (noni) on the immune system: Its molecular mechanisms of action. J Ethnopharmacol 115: 502–506.
Su BN, Pawlus AD, Jung HA, Keller WJ, McLaughlin JL, Kinghorn AD. Chemical Constituents of the Fruits of Morinda citrifolia (Noni) and Their Antioxidant Activity. Journal of natural products. 2005; 68(4):592-5.
Pradhan A, Jain P, Pal M, Chauhan M, Jain DK. Qualitative and quantitative determination of phytochemical contents of hydroalcoholic extract of Salmaliamalabarica. Pharmacologyonline 2019; 1:21-6.
Dutta R, Sharma MK, Khan A, Jha M. Phytochemical and in vitro antioxidant assay of Fumaria officinalis leaf extract. Journal of Advanced Scientific Research 2020; 11(03):176-82.
Jain DK, Gupta S, Jain R, Jain N. Anti-inflammatory Activity of 80% Ethanolic Extract of Acorus calamus Linn. Leaves in Albino Rats. Research Journal of Pharma Technology 2010; 3(3): 882-884.
Ali, M. S., Amin, M. R., Kamal, C. M. I., & Hossain, M. A.. In vitro antioxidant, cytotoxic, thrombolytic activities and phytochemical evaluation of methanol extract of the A. philippense L. leaves. Asian Pacific Journal of Tropical Biomedicine 2013. 3(6), 464-469.
Guideline Document on Acute oral Toxicity Testing, Series on Testing and Assessment No. 423. Paris: Organization for Economic Co-Operation and Development, OECD Environment, Health and Safety Publications; 1996. Available from: http//www.oecd.org/ehs.
Jonsson M, Jestoi M, Nathanail AV, Kokkonen UM, Anttila M, Koivisto P, Peltonen K. Application of OECD Guideline 423 in assessing the acute oral toxicity of moniliformin. Food Chem Toxicol. 2013; 53: 27-32.
Newbould BB: Chemotherapy of arthritis induced in rats by injection of Mycobacterial adjuvant. Br J PharmacolChemother. 1963, 21: 127-137.
Patel P, Patel D, Patel N. Experimental investigation of the anti-rheumatoid activity of Pleurotus sajorcaju in adjuvant-induced arthritic rats. Chin J Nat Med. 2012; 10:269e274.
Pathak N, Gohil P, Patel NB, Kasture S, Jivani N, Bhalodia Y. Curative effect of Albizia lebbeckmethanolic extract against adjuvant arthritis – with special reference to bone erosion. Int J Pharma Sci Drug Res 2009;1:183-7.
Singh S, Majumdar DK. Effect of fixed oil of Ocimum sanctum against experimentally induced arthritis and joint edema in laboratory animals. Int J Pharmacog1996;34:218-22.
Yende SR, Sannapuri VD, Vyawahare NS, Harle UN. Antirheumatoid activity of aqueous extract of Piper nigrum on Freund’s Adjuvant-induced arthritis in rats. Int J Pharma Sci Res 2010;1:129-33.
Bauerová K, Bezek A. Role of reactive oxygen and nitrogen species in etiopathogenesis of rheumatoid arthritis. Gen PhysiolBiophys1999;18:15-20.
Gelderman KA, Hultqvist M, Olsson LM, Bauer K, Pizzolla A, et al. (2007) Rheumatoid arthritis: the role of reactive oxygen species in disease development and therapeuticstrategies.Antioxid Redox Signal 9: 1541-1567.
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