Pollen Spectrum and Bee Flora Diversity in Northern Balochistan, Pakistan: A Melissopalynological Study
Keywords:
Melissopalynology, Pollen Spectrum, Bee Forage Plants, Honey Floral Origin, Multifloral Honey,, Apicultural, Sustainable BeekeepingAbstract
Melissopalynology, the study of pollen grains in honey, is a valuable tool for identifying floral resources exploited by bees and for determining the botanical and geographical origin of honey. This study aimed to document the diversity of bee forage plants in Northern Balochistan, Pakistan, and to highlight the region’s potential for sustainable apiculture. Five fresh honey samples were collected from local beekeepers in Ziarat, Loralai, Khanozai, Quetta, and Muslimbagh, along with representative plant specimens from the surrounding habitats. Pollen analysis was performed using the acetolysis method, and slides were prepared from floral anthers and honey samples to identify pollen taxa. All honey samples were classified as multifloral. A total of 30 pollen types representing multiple plant families were identified. The dominant taxa included Sonchus oleraceus, Peganum harmala, Helianthus annuus, Malus pumila, and Punica granatum. The family Asteraceae was the most prominent, being consistently present across all honey samples. Seasonal variation in pollen diversity was also observed, with higher representation of taxa during peak flowering periods. The pollen spectrum corresponded closely with the surrounding vegetation, confirming the reliability of melissopalynological analysis. The results demonstrate that the honey of Northern Balochistan is of multifloral origin and reflects the rich floral diversity of the region. These findings highlight the ecological significance of native plant species in sustaining honeybee populations and underscore the potential for developing apiculture as a sustainable livelihood. Conservation of key forage plants is recommended to support both ecological balance and local beekeeping practices
Downloads
Metrics
References
Adgaba, N., Al-Ghamdi, A., Tadesse, Y., Getachew, A., Awad, A. M., Ansari, M. J., … Alqarni, A. S. (2017). Nectar secretion dynamics and honey production potentials of some major honey plants in Saudi Arabia. Saudi Journal of Biological Sciences, 24(1), 180–191. https://doi.org/10.1016/j.sjbs.2016.02.010
Al-Khalifa, A. S., & Al-Arify, I. A. (1999). Physicochemical characteristics and pollen spectrum of some Saudi honeys. Food Chemistry, 67(1), 21–25. https://doi.org/10.1016/S0308-8146(99)00105-3
Gyawali, A. (2025). Evaluating the status and potential of honey production in Nepal. Insight from pollen analysis and beekeeping practices.
Junaid, M., & Khan, S. (2020). Problems and Prospects of Honey Value chain in Peshawar, Khyber Pakhtunkhwa: A Grounded Theory Analysis. Journal of Managerial Sciences, 14.
Kausar, G., Yousaf, A. I., Afzal, U., Tariq, W., Ambreen, M., Gull, Z., ... & Khan, S. U. (2024). Quality Assessment and Authentication of Raw Honey-Available at Local Markets of Punjab. Indus Journal of Bioscience Research, 2(02), 1038-1046.
Khan, K., Ahmad, M., Ali, M., Zafar, M., Haq, I. U., Papini, A., ... & Malik, K. (2022). Melissopalynological and biochemical profile of honeybee (Apis mellifera L.) flora in Southern Khyber Pakhtunkhwa, Pakistan. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, 156(5), 1177-1186.
Louveaux, J., Maurizio, A., & Vorwohl, G. (1978). Methods of melissopalynology. Bee World, 59(4), 139–157. https://doi.org/10.1080/0005772X.1978.11097714
Persano Oddo, L., & Piro, R. (2004). Main European unifloral honeys: Descriptive sheets. Apidologie, 35(S1), S38–S81. https://doi.org/10.1051/apido:2004049
Potts, S. G., Biesmeijer, J. C., Kremen, C., Neumann, P., Schweiger, O., & Kunin, W. E. (2010). Global pollinator declines: Trends, impacts and drivers. Trends in Ecology & Evolution, 25(6), 345–353. https://doi.org/10.1016/j.tree.2010.01.007
Shah, F. A., Akbar, W., Ullah, H., & Ullah, R. (2020). Floral resources and honeybee foraging in Balochistan, Pakistan. Journal of Entomology and Zoology Studies, 8(1), 350–356.
Song, X. Y., Yao, Y. F., & Yang, W. D. (2012). Pollen analysis of natural honeys from the central region of Shanxi, North China. PloS one, 7(11), e49545.
Qaiser, M., Perveen, A., Alam, J., Ali, H., & Abid, R. (2025). Asteraceae in Pakistan and Kashmir with special emphasis on distribution pattern and endemism. Genetic Resources and Crop Evolution, 1-30.
Anklam, E. (1998). A review of the analytical methods to determine the geographical and botanical origin of honey. Food Chemistry, 63(4), 549–562. https://doi.org/10.1016/S0308-8146(98)00057-0
Alam, W., Khan, Q. A., Ali, H. W., Kamal, D., Bibi, I., & Rizwan, M. (2025). Identification of the Melissopalynological Flora of Northern Punjab using Honey Bee Samples. Indus Journal of Bioscience Research, 3(5), 872-882.
Escuredo, O., Rodríguez-Flores, M. S., Míguez, M., & Seijo, M. C. (2023). Multivariate statistical approach for the discrimination of honey Samples from Galicia (NW Spain) using physicochemical and pollen parameters. Foods, 12(7), 1493.
Di Pasquale, G., Salignon, M., Le Conte, Y., Belzunces, L. P., Decourtye, A., Kretzschmar, A., ... & Alaux, C. (2013). Influence of pollen nutrition on honey bee health: Do pollen quality and diversity matter? PLoS ONE, 8(8), e72016. https://doi.org/10.1371/journal.pone.0072016
Haldhar, S. M., Nidhi, C. N., Singh, K. I., & Devi, A. S. (2021). Honeybees diversity, pollination, entrepreneurship and beekeeping scenario in NEH region of India. Journal of Agriculture and Ecology, 12, 27-43.
Tulu, D., Aleme, M., Mengistu, G., Bogale, A., Bezabeh, A., & Mendesil, E. (2023). Melissopalynological analysis and floral spectra of Apis mellifera scutellata Lepeletier bees in different agroecologies of southwest Ethiopia. Heliyon, 9(5).
Essien, B. C., & Olaniyi, B. O. (2023). Pollen analysis and physicochemical characterization of honey samples from owo local government area, Ondo State, Nigeria. Global Research in Environment and Sustainability, 1(7), 66-79.
Louveaux, J., Maurizio, A., & Vorwohl, G. (1978). Methods of melissopalynology. Bee World, 59(4), 139–157. https://doi.org/10.1080/0005772X.1978.11097714
Bibi, S., Husain, S. Z., & Malik, R. N. (2008). Pollen analysis and heavy metals detection in honey samples from seven selected countries. Pak J Bot, 40(2), 507-516.
Odoux, J. F., Feuillet, D., Aupinel, P., Loublier, Y., Tasei, J. N., & Mateescu, C. (2012). Territorial biodiversity and consequences on physico-chemical characteristics of honey. Journal of Apicultural Research, 51(4), 1–10. https://doi.org/10.3896/IBRA.1.51.4.11
Pérez-Arquillué, C., Conchello, P., Arino, A., Juan, T., & Herrera, A. (1995). Physicochemical attributes and pollen spectrum of Spanish honeys. Food Chemistry, 54(2), 167–172. https://doi.org/10.1016/0308-8146(95)00011-R
Ahmad, S., Zafar, M., Ahmad, M., Zafar, S., Arfan, M., Khan, A. M., ... & Majeed, S. (2023). Melissopalynological studies of autumn honey samples from khyber pakhtunkhwa, pakistan. J Anim Plant Sci-JAPS, 33(5), 1184-1192.
Jamil Noor, M., Ahmad, M., Ashraf, M. A., Zafar, M., & Sultana, S. (2016). A review of the pollen analysis of South Asian honey to identify the bee floras of the region. Palynology, 40(1), 54-65.
Villanueva-G, R., Roubik, D. W., & Colli-Ucán, W. (2005). Extinction of Melipona beecheii and traditional beekeeping in the Yucatán peninsula. Bee World, 86(2), 35-41.
Alam, W., Khan, Q. A., Ali, H. W., Kamal, D., Bibi, I., & Rizwan, M. (2025). Identification of the Melissopalynological Flora of Northern Punjab using Honey Bee Samples. Indus Journal of Bioscience Research, 3(5), 872-882.
Downloads
Published
How to Cite
Issue
Section
License

This work is licensed under a Creative Commons Attribution 4.0 International License.
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
Terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.